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Alien Uprising: Zothren Invasion, a standalone game in the Alien Uprising family, is played in two phases. During the first phase, the players go through a card-drafting process to build robots which will be used to fight off an alien invasion. Each robot will have six body parts, each with various weapons and armor. Once the robots are fully constructed, the Zothren Invasion begins.

Players work in a voluntary co-op fashion to defend earth from the Zothren. If all of the robots are destroyed by the Zothren, humanity is lost. However, if the requisite number of aliens are successfully defeated, then the player who controls the robot that has the accumulated the most Zothren losses is the victor.